Determination of verbascoside in the root of Rehmannia glutinosa varieties 19 by high performance liquid chromatography
Abstract
This study aimed to validate a procedure for the quantification of verbascoside in the root of Rehmannia glutinosa varieties 19 by high performance liquid chromatography (HPLC) to serve the assessment of medicinal quality. The results have selected suitable chromatographic conditions, using the gemini C18 column (250 × 4.6 mm, 5 µm), detector at 334 nm, mobile phase acetonitrile - phosphoric acid 0.1%, and the flow rate at 0.8 ml/min. Peak area and verbascoside concentration are strongly correlated (r = 0.9997), Y = 2349X + 7259.6 . The procedure has good accuracy and repeatability with RSD < 2%. This procedure was applied to quantify verbascoside in the root of Rehmannia glutinosa varieties 19 , which is grown in Vinh Phuc, Phu Tho provinces, and the results determination of verbascoside were 0.027% and 0.028%, respectively.