Detection of tobacco mosaic virus and cucumber mosaic virus on Rehmannia glutinosa variety 19 by Enzyme-Linked Immunosorbent Assay
Abstract
Tobacco Mosaic Virus ( TMV) and Cucumber Mosaic Virus (CMV) are common pathogens of Rehmannia glutinosa Libosch. The study conducted experiments to detect TMV and CMV viruses by Enzyme-Linked Immunosorbent Assay ( ELISA ) on the mother plants sampled for propagation to eliminate pathogens from the initial sampling stage . The detection results of the stem, tuberous roots, primary shoots, and in vitro seedlings of Rehmannia glutinosa variety 19 (RG19) showed that the samples were not infected with two types of TMV and CMV viruses. This is the starting material for propagating disease-free seedlings to create a highly qualified source of in vitro RG19 for crops.